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af2254  (R&D Systems)


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    Structured Review

    R&D Systems af2254
    Af2254, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dner/Mouse+DNER+Antibody/pmc12821072-6-0-4
    Average 93 stars, based on 21 article reviews
    af2254 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: DNER and NFIA are Expressed by Developing and Mature AII Amacrine Cells in the Mouse Retina
    Article Snippet: .. The antibody used to detect DNER (1:500; #AF2254; R&D Systems, Minneapolis, MN; RRID:AB_355202) was a goat polyclonal IgG raised against recombinant mouse DNER (aa26–637) derived from the mouse myeloma cell line NS0, which was confirmed by the manufacturer to detect mouse DNER in ELISA and Western blot assays, as well as label Purkinje cells of the mouse cerebellum, as expected. ..

    Derivative Assay:

    Article Title: DNER and NFIA are Expressed by Developing and Mature AII Amacrine Cells in the Mouse Retina
    Article Snippet: .. The antibody used to detect DNER (1:500; #AF2254; R&D Systems, Minneapolis, MN; RRID:AB_355202) was a goat polyclonal IgG raised against recombinant mouse DNER (aa26–637) derived from the mouse myeloma cell line NS0, which was confirmed by the manufacturer to detect mouse DNER in ELISA and Western blot assays, as well as label Purkinje cells of the mouse cerebellum, as expected. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: DNER and NFIA are Expressed by Developing and Mature AII Amacrine Cells in the Mouse Retina
    Article Snippet: .. The antibody used to detect DNER (1:500; #AF2254; R&D Systems, Minneapolis, MN; RRID:AB_355202) was a goat polyclonal IgG raised against recombinant mouse DNER (aa26–637) derived from the mouse myeloma cell line NS0, which was confirmed by the manufacturer to detect mouse DNER in ELISA and Western blot assays, as well as label Purkinje cells of the mouse cerebellum, as expected. ..

    Western Blot:

    Article Title: DNER and NFIA are Expressed by Developing and Mature AII Amacrine Cells in the Mouse Retina
    Article Snippet: .. The antibody used to detect DNER (1:500; #AF2254; R&D Systems, Minneapolis, MN; RRID:AB_355202) was a goat polyclonal IgG raised against recombinant mouse DNER (aa26–637) derived from the mouse myeloma cell line NS0, which was confirmed by the manufacturer to detect mouse DNER in ELISA and Western blot assays, as well as label Purkinje cells of the mouse cerebellum, as expected. ..

    other:

    Article Title: Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function
    Article Snippet: The following antibodies were used in this study: ADAM10 (Abcam/Epitomics, Cambridge, UK, EPR5622), Actin (Sigma-Aldrich, A2066), sAPPα 5G11 , sAPPβ 8C10 , APP 22C11 (Millipore, Billerica, MA), MAB348sAPPα (Covance, Princeton, NJ, Sig-39151), APP (Sigma-Aldrich, A8717), Cadherin 6 (Abcam/Epitomics, EPR217), Calnexin (Enzo, Stressgen, Farmingdale, NY, USA, ADI-SPA-860), Calbindin, (rabbit polyclonal anti-calbindin D-28k antibodies Swant, Bellinzona, Switzerland), mContactin (R&D Systems, Wiesbaden, Germany, AF4439), DNER (R&D Systems, AF2254), GFAP (Sigma-Aldrich, G3893), L1CAM Clone 555 (kindly provided by Peter Altevogt), LDL receptor (R&D systems, AF2255), Lrrc4b (R&D systems, AF4995), MT4-MMP (MMP17), (Abcam/Epitomics, EP1270Y), N-Cadherin (Abcam/Epitomics, EPR1791-4), NrCAM (Abcam, ab24344), Neuroligin-1 (Synaptic Systems, Göttingen, Germany 4C12), Synaptophysin (mouse monoclonal Sigma-Aldrich).

    Immunohistochemistry:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: The following antibodies were validated for flow cytometry of mouse cells by the manufacturer using respective positive control cells (e.g. mouse splenocytes for CD45) compared to isotype control antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119), CD24-APC (eBioscience 17-0242-82, clone M1/69), F4/80-PE-Cy7 (BioLegend 123114, clone BM8), EpCAM-APC (eBioscience 17-5791-82, clone G8.8), CD45-Pacific Blue (BioLegend 103126, clone 30-F11), CD31-Pacific Blue (BioLegend 102422, clone 390), TER-119-Pacific Blue (BioLegend 116232, clone TER-119), CD24-PE-Cy7 (Biolegend 101821, clone M1/69), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4). .. The following antibodies were validated by manufacturer for IHC /Immunoassay using positive control tissue sections, positive control cell line lysates, gene knockout/knockdown and overexpression experiments: Hes1 (Cell signaling technology 11988), Yap(D8H1X)XP® (Cell signaling technology 14074S), CC10/Scgb1a1 (Santa Cruz sc-9772), Syp (Neuromics MO20000), CC10/Scgb1a1 (Millipore 07-623), RFP/Tomato (Biosource MBS448092), CGRP (Sigma-Aldrich C8198), BrdU (BD Biosciences 347580), Dner (R&D Systems AF2254), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313), AGER/RAGE (R&D Systems MAB1179), ASCL1 (Santa Cruz sc-374104), NOTCH2 (Cell Signaling Technology 5732), UCHL1 (Sigma-Aldrich HPA005993), and HSP90 (Cell Signaling Technology 4877). ..

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: 3 n atu re research | rep o rtin g su m m ary A p ril2020 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) FACS antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100), EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100), CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-PE-Cy7 (Biolegend 101821, clone M1/69, 1:200), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4, 1:100), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220, 1:50), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. IHC antibodies: Hes1 (Cell signaling technology 11988, 1:200), Yap(D8H1X)XP® (Cell signaling technology 14074S, 1:200), CC10/Scgb1a1 (Santa Cruz sc-9772, 1:50), Syp (Neuromics MO20000), 1:200, CC10/Scgb1a1 (Millipore 07-623, 1:200), RFP/Tomato (Biosource MBS448092, 1:500), CGRP (Sigma-Aldrich C8198, 1:200), BrdU (BD Biosciences 347580, 1:50), Dner (R&D Systems AF2254, 1:50), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313, 1:200), AGER/RAGE (R&D Systems MAB1179, 1:200), Alexa Fluor®488 donkey anti-goat IgG (Invitrogen A-11055, 1:200), Alexa Fluor®488 donkey anti-rabbit IgG (Invitrogen A-21206, 1:200), Alexa Fluor®594 donkey antirabbit IgG (Invitrogen A-21207, 1:200), Alexa Fluor®594 donkey anti-goat IgG (Invitrogen A-11058, 1:200), Alexa Fluor®594 donkey anti-rat IgG (Invitrogen A-21209, 1:200) and Alexa Fluor®488 donkey anti-mouse IgG (Invitrogen A-21202, 1:200). .. ChIP antibodies: Yap(D8H1X)XP® (Cell signaling technology 14074S, 0.14ug of antibody per 10 million cells) and Rest antibody (Millipore 17-641, 4ug of antibody per 10 million cells).

    Positive Control:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: The following antibodies were validated for flow cytometry of mouse cells by the manufacturer using respective positive control cells (e.g. mouse splenocytes for CD45) compared to isotype control antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119), CD24-APC (eBioscience 17-0242-82, clone M1/69), F4/80-PE-Cy7 (BioLegend 123114, clone BM8), EpCAM-APC (eBioscience 17-5791-82, clone G8.8), CD45-Pacific Blue (BioLegend 103126, clone 30-F11), CD31-Pacific Blue (BioLegend 102422, clone 390), TER-119-Pacific Blue (BioLegend 116232, clone TER-119), CD24-PE-Cy7 (Biolegend 101821, clone M1/69), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4). .. The following antibodies were validated by manufacturer for IHC /Immunoassay using positive control tissue sections, positive control cell line lysates, gene knockout/knockdown and overexpression experiments: Hes1 (Cell signaling technology 11988), Yap(D8H1X)XP® (Cell signaling technology 14074S), CC10/Scgb1a1 (Santa Cruz sc-9772), Syp (Neuromics MO20000), CC10/Scgb1a1 (Millipore 07-623), RFP/Tomato (Biosource MBS448092), CGRP (Sigma-Aldrich C8198), BrdU (BD Biosciences 347580), Dner (R&D Systems AF2254), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313), AGER/RAGE (R&D Systems MAB1179), ASCL1 (Santa Cruz sc-374104), NOTCH2 (Cell Signaling Technology 5732), UCHL1 (Sigma-Aldrich HPA005993), and HSP90 (Cell Signaling Technology 4877). ..

    Knock-Out:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: The following antibodies were validated for flow cytometry of mouse cells by the manufacturer using respective positive control cells (e.g. mouse splenocytes for CD45) compared to isotype control antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119), CD24-APC (eBioscience 17-0242-82, clone M1/69), F4/80-PE-Cy7 (BioLegend 123114, clone BM8), EpCAM-APC (eBioscience 17-5791-82, clone G8.8), CD45-Pacific Blue (BioLegend 103126, clone 30-F11), CD31-Pacific Blue (BioLegend 102422, clone 390), TER-119-Pacific Blue (BioLegend 116232, clone TER-119), CD24-PE-Cy7 (Biolegend 101821, clone M1/69), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4). .. The following antibodies were validated by manufacturer for IHC /Immunoassay using positive control tissue sections, positive control cell line lysates, gene knockout/knockdown and overexpression experiments: Hes1 (Cell signaling technology 11988), Yap(D8H1X)XP® (Cell signaling technology 14074S), CC10/Scgb1a1 (Santa Cruz sc-9772), Syp (Neuromics MO20000), CC10/Scgb1a1 (Millipore 07-623), RFP/Tomato (Biosource MBS448092), CGRP (Sigma-Aldrich C8198), BrdU (BD Biosciences 347580), Dner (R&D Systems AF2254), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313), AGER/RAGE (R&D Systems MAB1179), ASCL1 (Santa Cruz sc-374104), NOTCH2 (Cell Signaling Technology 5732), UCHL1 (Sigma-Aldrich HPA005993), and HSP90 (Cell Signaling Technology 4877). ..

    Over Expression:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: The following antibodies were validated for flow cytometry of mouse cells by the manufacturer using respective positive control cells (e.g. mouse splenocytes for CD45) compared to isotype control antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119), CD24-APC (eBioscience 17-0242-82, clone M1/69), F4/80-PE-Cy7 (BioLegend 123114, clone BM8), EpCAM-APC (eBioscience 17-5791-82, clone G8.8), CD45-Pacific Blue (BioLegend 103126, clone 30-F11), CD31-Pacific Blue (BioLegend 102422, clone 390), TER-119-Pacific Blue (BioLegend 116232, clone TER-119), CD24-PE-Cy7 (Biolegend 101821, clone M1/69), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4). .. The following antibodies were validated by manufacturer for IHC /Immunoassay using positive control tissue sections, positive control cell line lysates, gene knockout/knockdown and overexpression experiments: Hes1 (Cell signaling technology 11988), Yap(D8H1X)XP® (Cell signaling technology 14074S), CC10/Scgb1a1 (Santa Cruz sc-9772), Syp (Neuromics MO20000), CC10/Scgb1a1 (Millipore 07-623), RFP/Tomato (Biosource MBS448092), CGRP (Sigma-Aldrich C8198), BrdU (BD Biosciences 347580), Dner (R&D Systems AF2254), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313), AGER/RAGE (R&D Systems MAB1179), ASCL1 (Santa Cruz sc-374104), NOTCH2 (Cell Signaling Technology 5732), UCHL1 (Sigma-Aldrich HPA005993), and HSP90 (Cell Signaling Technology 4877). ..



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